Hong Fan

       Dept. of Genetic and Developmental Biology,  

              Medical School of Southeast University

        Key Laboratory of Developmental Genes and Human Diseases, 

        Ministry of Education, Southeast University

         87 Dingjiaqiao Road, Nanjing, 210009, P. R. China

          Tel:    +86-025-83272370 (O)  Fax : +86-025-83272370

        E-mail:   fanh@seu.edu.cn

 

 

 

EDUCATION

 

09/1985 - 07/1990       B.Sc.                     Medicine, Medical School of Jiamusi University,

Jiamusi, China

09/1992 - 07/1995       M.Sc.                     Medical Genetics, Medical School of Jiamusi University,

Jiamusi, China

09/1999 - 09/2002       Ph.D.                     Medical Genetics, Harbin Medical University,

Harbin, China

09/2002 - 10/2004      Postdoc. Fellow    Medical School of Southeast University,

                                                                  Nanjing, China

09/2006 - 12/2006     Visiting Scholar    Medical School of Magdeburg University, Magdeburg, Germany

        03/2008 – 09/2008    Visiting Scholar    Medical School of Hong Kong University, Hong Kong, China

 

APPOINTMENTS:

04/2009 - to date: Professor.

Director of Department of Genetic and Developmental, Medical School of Southeast University. Jiangsu, China

 

10/2004 – 04/2009 Associate Professor.

Medical School of Southeast University. Jiangsu, China

 

 07/1995—09/1999: Research Associate 

Jiamusi Medical College, Heilongjiang , China

 

07/1990—09/1992: Research Assistant  

Jiamusi Medical College, Heilongjiang , China

 

LABORATORY TECHNIQUES AND SKILLS

1. Molecular Genetics

(1)   RFLP and its related techniques, PCR, PCR-SSCP, PCR-SSLP, multi-PCR, RT-PCR, MSP, etc.

(2)   DNA sequencing, Cloning of DNA segment

(3)   Electrophoresis (agarose gel electrophoresis, PAGE, SDS -PAGE)

(4)   Construction and screening of genomic DNA, cDNA libraries and subtractive hybridization cDNA library.

(5)   Southern blot, Northern blot, Western blot and ASO, Dot and Transfection in situ hybridization.

(6)   Designed of siRNA sequence and prepared of siRNA construct

 

2. Cell Culture

(1)   Establishment of immortal lymphoblastoid cell lines

(2)   Culture of tumor cell lines such as hepatocellular carcinoma cell lines, gastric cancer cell line.

(3)   Short-term culture of peripheral blood lymphocyte cell

 

3. Cytogenetics

(1)   Lymphocyte metaphase preparation and analysis of karyotype

(2)   Preparation and analysis of routine G-, C- and R-banded as well as high resolution G- banded chromosomes from cultured cells, especially from primary solid tumor cells

(3)   Preparation and analysis of differentiated staining of sister chromatics

(4)   Analysis of human chromosome fragile sites

(5)   FISH (centromere FISH, single copy FISH, chromosome painting, etc.)

 

OTHER SKILLS

1.       Lectures to undergraduate and graduate students (involving on-site instruction, grading and lab technique training).  Medical Genetics, Medical Biology and Cell Biology

2.       Competent computing skills and knowledge in word and data processing, statistical analysis and experiment design

 

MEMBERSHIP OF ACADEMIC ASSOCIATIONS

1.     Member of the Chinese Genetics Association

2.     Member of the Chinese Cell Biology Association

3.     Member of the Jiangsu developmental biology Association

 

RESEARCH interests:

My research is focused on the role of DNA methyltransferases on silenced tumor suppressor genes and the epigenetic modifications (DNA methylation and histone modification) in malignancies. We try to find epigenetic biomarker to diagnosis on hepatocellular carcinoma or gastric cancer.  My research is also focused on the association study with tumor and DNMT3 family tagSNPs. Recently, my lab devotes to explore the epigenetic regulation on the dedifferentiation of gastric cancer.

 

grants:

1.     NO. 30470950    (Fan)  01/01/2005 – 31/12/2005

Supported by National Natural Science Foundation of China.

Study on DNMTs epigenetic mechanism in hepatocellualar carcinogensis.

 

2.     NO. 30971605: (Fan)  01/01/2009 – 31/12/2012

Supported by National Natural Science Foundation of China.

Research on HBx regulate DNMTs in inactivating Tumor suppressor genes in hepatocellularcarcinoma.

 

3.     NO. 81171915: (Fan)  01/01/2012 – 31/12/2015

Supported by National Natural Science Foundation of China.

DNMT3A variants biological function and its epigenetic regulation in gastric carcinogenesis.

 

4.      NO.91229107 (Fan)  2013,1，1—2015,12,31

HBX activated lncRNA mediated transformation from Chronic hepatitis to hepatocellular carcinoma

 

PUBLICATIONS

“Original” paper   (* Corresponding author )

(1)  Wu HZ, Zhang K, Pihai Gong PH, Qiao FC, Wang L, Cui H, Sui XY, Gao JF, Fan H*A novel functional tagSNP rs7560488 in the DNMT3A1 promoter is associated with susceptibility to gastric cancer by modulating promoter activity. PLOS ONE, 2014, In press

(2)  Qiu XM, Zhang LH, Lu S, Song YW, Lao Y B, Hu JJ, Fan H* . Upregulation of DNMT1 mediated by HBx suppresses RASSF1A expression independent of DNA methylation. Oncol Rep. 2014；31: 202-208

(3)  Lao YB, Wu HZ, Zhao CC, Wu QY, Qiao FC, Fan H*. Promoter polymorphisms of DNA methyltransferase 3B and risk of hepatocellular carcinoma. Biomedical Reports. 2013;1: 771-775.

(4)  Zhao CC, Yan F, Wu HZ, Qiao FC, Qiu XM, Fan H*. DNMT3A -448A>G polymorphism and the risk for hepatocellular carcinoma. Biomedical Reports. 2013;1:664-668.

(5)  Qiu X, Dong S, Qiao F, Lu S, Song Y, Lao Y, Li Y, Zeng T, Hu J, Zhang L, Zhang L, Fan H*. HBx-mediated miR-21 upregulation represses tumor-suppressor function of PDCD4 in hepatocellular carcinoma.Oncogene. ; 32, 3296 – 3305

(6)  Wu Q, Lu S, Wang L, Hu J, Qiao F, Qiu X, Zhao C, Lao Y, Song Y, Fan H*. DNMT3A rs36012910 A>G polymorphism and gastric cancer susceptibility in a Chinese population. Mol Biol Rep.2012 Dec;39(12):10949-55.

(7)  Fan H*, Chen L, Zhang F, Quan Y, Su X, Qiu X, Zhao Z, Kong KL, Dong S, Song Y, Chan TH, Guan XY. MTSS1, a novel target of DNA methyltransferase 3B, functions as a tumor suppressor in hepatocellular carcinoma.Oncogene. 2011 Sep 12. doi: 10.1038/onc.2011.411. [Epub ahead of print]

(8)  Yang J, Wei X, Wu Q, Xu Z, Gu D, Jin Y, Shen Y, Huang H, Fan H, Chen J. Clinical significance of the expression of DNA methyltransferase proteins in gastric cancer. Mol Med Report.2011 Nov-Dec;4(6):1139-43.

(9)  Huang H, Wei X, Su X, Qiao F, Xu Z, Gu D, Fan H, Chen J.  Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer. Int J Oncol. 2011;38(6):1557-64. 

(10)          Zhao ZJ,Wu QX,Chen J, Qiu XM,Zhang JQ, Fan H*.  Depletion of DNMT3A suppressed cell proliferation and restored PTEN in hepatocellular carcinoma cells. J Biomed Biotech. 2010;2010:737535.

(11)           Fan H*Liu DS, Qiu XM, Qiao FC, Wu QX, Su XW, Zhang F, Song YW, Zhao ZJ, Xie W  A functional polymorphism in the DNA methyltransferase 3A promoter modifies the susceptibility in gastric cancer but not in esophageal carcinoma. BMC Medicine. 2010 ；Feb 3;8(1):12

(12)          Su XW, Lv CY, Qiao FC, Qiu XM, Huang WB, Wu QX, Zhao ZJ, Fan H*.  Expression pattern and clinical significance of DNA methyltransferase 3B variants in gastric carcinoma. Oncol Rep. 2010; 23:819-26.

(13)          Hu J, Fan H*, Liu D, Zhang S, Zhang F, Xu H. DNMT3B promoter polymorphism and risk of gastric cancer. Dig Dis Sci.2010;55(4):1011-6.

(14)          Qiu XM, Qiao FC, Su XW, Zhao ZJ, Fan H*. Epigenetic activation of E-cadherin is a candidate therapeutic target in human hepatocellular carcinoma. Exp. Ther. Med. 2010;1: 519-523.

(15)          Fan H*, Zhao ZJ, Cheng J, Su XW, Wu QX, Shan YF. Overexpression of DNA methyltransferase 1 and its biological significance in primary hepatocellular carcinoma. World J Gastroenterol. 2009 Apr 28;15(16):2020-6.

(16)          Fan H*, Liu DS, Zhang SH, Hu JB, Zhang F, Zhao ZJ.The DNMT3B G>T promoter Polymorphism and the Risk of Esophagus Carcinoma in a Chinese Population. World J Gastroenterol. 2008 Apr 14;14(14):2230-4.

(17)          Fan H*, Zhang F, Hu J, Liu D, Zhao Z.Promoter polymorphisms of DNMT3B and the risk of colorectal cancer in Chinese: a case-control study. J Exp Clin Cancer Res. 2008; 27:24.

(18)          Fan H*, Cheng J, Zhao ZJ.  Inhibition of de novo DNA Methyltransferase 3B is a Potential. Therapy for Hepatocellular Carcinoma.  Gastroen Res. 2008;1:33-39

(19)          Fan H*, Zhao Z, Quan Y, Xu J, Zhang J, Xie W.  DNA methyltransferase 1 knock down induces silenced CDH1 gene re-expression by demethylation of methylated CpG in HCC cell line SMMC-7721. Eur J Gastroenterol Hepatol. 2007;19:952-61

(20)          Fan H, Zhao ZJ, Cheng YC, Shan YF, Lu ZH, Zhang JQ, Xie W*. Analysis of gene induction and apoptosis in human hapetocellular carcinoma cells SMMC-7721 exposed to the methylation inhibitor 5-Aza-2’-deoxycytidine.  Chin Med J (Engl). 2007;120:1626-31

(21)          Xu W, Fan H*, He X. LOI of IGF2 is associated with esophageal cancer and linked to methylation status of IGF2 DMR. J Exp Clin Cancer Res. 2006;25: 543-7. 

 

Abstracts/conference papers:

1.      Fan H, Xu J, Wei SW, Zhao ZJ, Zhang JQ, Xie W*. RNA Interference of DNMT1 Inhibits Cell Proliferation in Hepatocellular Carcinoma Cell Lines. “2004 Asian-Pacific Conference of Tumor Biology and Medicine” Xian, 2004,8

2.      Fan H, Xu J, Zhao ZJ, Zhang JQ, Xie W*. Decreased DNMT1 inducing the demethylation of CpG islands and restoration of aberrant gene expression silenced in human tumor.  Cell Res. 2003, 13: 425-426

3.      Fan H, Li Yu,Feng huichen, et al. Cloning and analysing the metastasis related genes from human lung Adencarcinoma cell lines.Asian-Pacific Conference of Tumor Biology and Medicine Beijing, 2001,9