研究内容

1、双微体超微结构研究；

2、不同组织来源实体肿瘤中双微体扩增子的起源、序列及结构比较分析；

3、双微体携带扩增基因功能及相关信号通路；

4、实体肿瘤耐药性获得过程中双微体的形成和演化机制研究。

 

发表学术文章

1. Meng XN, Ma JF, Liu YH, Li SQ, Wang X, Zhu J, Cai MD, Zhang HS, Song T, Xing S, Hou LQ, Guo H, Cui XB, Han J, Liu P, Ji GH, Sun WJ, Yu JC, Fu SB*. Dynamic genomic changes in methotrexate-resistant human cancer cell lines beyond DHFR amplification suggest potential new targets for preventing drug resistance. British Journal of Cancer. 2024 May;130(11):1819-1827.

2. Wang Y, Zou H, Ji W, Huang M, You B, Sun N, Qiao Y, Liu P, Xu L, Zhang X, Cai M, Kuang Y, Fu S, Sun W, Jia X, Wu J. Repression of the SUMO-conjugating enzyme UBC9 is associated with lowered double minutes and reduced tumor progression. CANCER BIOLOGY & THERAPY. 2024 Dec 31;25(1):2323768.

3. Wang X, Qu Y, Xing R, Zhou J, Liu Y, Zhang H, Zhu J, Ma J, Cui X, Song T, Xing S, Ji G, Liu P, Sun W, Fu S, Meng X. Novel insights into the ecDNA formation mechanism involving MSH3 in methotrexate‑resistant human colorectal cancer cells. Int J Oncol. 2023 Dec;63(6):134. doi: 10.3892/ijo.2023.5582. Epub 2023 Oct 27. PMID: 37888748; PMCID: PMC10631765.

4. Jia X, Guan R, Cui X, Zhu J, Liu P, Zhang L, Wang D, Zhang Y, Dong K, Wu J, Ji W, Ji G, Bai J, Yu J, Yu Y, Sun W, Zhang F, Fu S.Molecular structure and evolution mechanism of two populations of double minutes in human colorectal cancer cells. J Cell Mol Med. 2020 Dec;24(24):14205-14216.

5. Yu L, Zhao Yan, Quan C, Ji W, Zhu J, Huang Y, Guan R, Sun D, Jin Y, Meng X, Zhang C, Yu Y, Bai J, Sun W, and Fu S*. Gemcitabine eliminates double minute chromosomes from human ovarian cancer cells.Plos One. 2013 July 6 (Accepted)

6. Zhu J, Yu Y, Meng X, Fan Y, Zhang Y, Zhou C, Yue Z, Jin Y, Zhang C, Yu L, Ji W, Jia X, Guan R, Wu J, Yu J, Bai J, Guan XY, Wang M, Lee KY, Sun W, and Fu S*. (2013)De novo-generated small palindromes are characteristic of amplicon boundary junction of double minutes.Int J Cancer.133(4):797-806. PMID: 23382041

7. Jin Y, Liu Z, Cao W, Ma X, Fan Y, Yu Y, Bai J, Chen F, Rosales J, Lee KY, and Fu S*. (2012) Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.PLoS One. 7(2):e30419. PMID: 22319568

8. Fan Y, Mao R, Lv H, Xu J, Yan L, Liu Y, Shi M, Ji G, Yu Y, Bai J, Jin Y, and Fu S*. (2011) Frequency of double minute chromosomes and combined cytogenetic abnormalities and their characteristics.J Appl Genet. 52(1):53-9. PMID: 21107781

9. Kong F, Zhu J, Wu J, Peng J, Wang Y, Wang Q, Fu S, Yuan LL, and Li T. (2011) dbCRID: a database of chromosomal rearrangements in human diseases.Nucleic Acids Res. 39(Database issue):D895-900. PMID: 21051346

10. Fan Y, Mao R, Bai J, Zhang X, Lei Q, Fu S*.(2008) AFM images of G1-phase premature condensed chromosomes:Evidence for 30 nm changed to 50 nm chromatin fibers.Applied Surface Science. 254(6):1676-83.

11. Fan Y, Zhang X, Bai J, Mao R, Zhang C, Lei Q, Fu S*. (2007) Ultrastructural organization of premature condensed chromosomes at S-phase as observerd by atomic force microscopy.Applied Surface Science. 253(12):5281-6.

12. Zhang CY, Feng YX, Yu Y, Sun WJ, Bai J, Chen F, Fu SB*. (2006) The molecular mechanism of resistance to methotrexate in mouse methotrexate-resistant cells by cancer drug resistance and metabolism SuperArray.Basic Clin Pharmacol Toxicol. 99(2):141-5. PMID: 16918715

13. Deng X, Zhang L, Zhang Y, Yan Y, Xu Z, Dong S, and Fu S*. (2006) Double minute chromosomes in mouse methotrexate-resistant cells studied by atomic force microscopy.Biochem Biophys Res Commun, 346(4):1228-33. PMID: 16806082

14. Feng YX, Bai J, Zhang CY, Fu SB*. (2006) Proteomic analysis for the identification of proteins related to methotrexate resistance.Yi Chuan Xue Bao. 33(5):391-6. PMID: 16722333

15. 张钰，邓新宇，白静，李璞，傅松滨*. (2006)人卵巢癌细胞及小鼠MTX抗性细胞中DMs大小的测定.国际遗传学杂志, 29(4): 241-5.

16. 邓新宇，张钰，曾力，国立秋，闫永达，董申，张贵寅，李璞，傅松滨*.（2003）原子力显微镜在双微体形态学研究中的应用.遗传学报, 30(9):881-5. PMID: 14577382

17. 郭志涛, 武慧慧, 耿淑慧, 陈晨, 孟祥宁, 孙文靖, 朱静. 基于高通量测序技术对染色体外环状DNA研究的最新进展. 生命科学, 2023;35(6)：824-832.

18. 武慧慧, 耿淑慧, 郭志涛, 孟祥宁, 朱静. 染色体外环状DNA 的研究进展. 癌变 畸变 突变. 2023;35(5):392-396.